anti pag1  (Proteintech)

Journal: Frontiers in Pharmacology

Article Title: Decoding monocyte heterogeneity in sepsis: a single-cell apoptotic signature for immune stratification and guiding precision therapy

doi: 10.3389/fphar.2025.1675887

Figure Lengend Snippet: Development and validation of the nomogram-based predictive model (A) Nomogram diagnostic prediction model constructed using G0S2, ITM2A, PAG1, and GZMA. (B) Calibration curves assessing consistency between predicted and actual observed values. (C) Decision Curve Analysis (DCA) evaluating the clinical net benefit of the nomogram. The x-axis represents the threshold probability for diagnosing sepsis, while the y-axis represents the net benefit. The curve for our model (red line) shows a greater net benefit across a wide range of clinically relevant thresholds compared to the strategies of treating all patients (dashed gray line) or no patients (solid blue line), indicating its potential for clinical utility. (D) Clinical impact curve illustrating the practical application of the nomogram. At any given risk threshold on the x-axis, the red curve shows the number of individuals predicted to be high-risk, while the blue curve shows the number of true positive cases within that group. The close approximation of the two curves demonstrates the model’s strong performance in accurately identifying patients at high risk of sepsis in a clinical setting. (E–H) ROC curve analyses evaluating predictive accuracy of the model in the training and multiple validation datasets.

Article Snippet: The PVDF membranes were incubated overnight at 4 °C with primary antibodies targeting G0S2 (Abcam, ab236113; 1:1000), GZMA (Proteintech, 11288-1-AP; 1:1000), ITM2A (Proteintech, 14407-1-AP; 1:1000), and PAG1 (Proteintech, 25029-1-AP; 1:1000).

Techniques: Biomarker Discovery, Diagnostic Assay, Construct

Journal: International Journal of Molecular Sciences

Article Title: Identification of Key Ubiquitination Sites Involved in the Proteasomal Degradation of AtACS7 in Arabidopsis

doi: 10.3390/ijms25052931

Figure Lengend Snippet: The degradation of AtACS7 and AtACS7 K285RK366R . Degradations of AtACS7 K285RK366R and its wild-type control were examined in the cell-free degradation assays without ( A , B ) or with ( C , D ) MG132 treatment. The purified MBP-AtACS7-His or MBP-AtACS7 K285RK366R -His mutant protein was incubated with total protein extracted from 10-day-old light-grown Arabidopsis seedlings. The MBP-AtACS7-His and MBP-AtACS7 K285RK366R -His proteins at the indicated time points were determined by Western blot analysis using an anti-MBP antibody. Anti-PAG1 was used as a sample loading control. The intensity ratios between AtACS7 and the PAG1 band in each lane were normalized to time the point of 0 min (value set to 1) and are shown under the blots. Representative gel images of the three biological replicates are shown in ( A , C ), and the relative levels of MBP-AtACS7-His and MBP- AtACS7 K285RK366R -His are shown in ( B , D ). Error bars represent SD from three independent assays. (Student’s t test, * p < 0.05). min, minutes.

Article Snippet: The AtACS7 protein was detected using an anti-MBP antibody (NEB, Beijing, China) and PAG1 was detected using an anti-PAG1 antibody (Abcam, Cambridge, UK).

Techniques: Purification, Mutagenesis, Incubation, Western Blot